HIGH PERFORMANCE LIQUID CHROMATOGRAPH

Make:  Shimadzu, Japan
Model:  SPD-10AVp (Shimadzu)
Year of Installation:  2001


Technical Specifications:
Sl No.ItemSpecification
1.Pump typeDouble reciprocating pump
2.Number of solvents mixed2
3.Mix ratio setting range0–100% (in 0.1% steps)
4.Pumping methodsConstant flow delivery & Constant pressure delivery.
5.Flow rate setting range0.1–9.999 mL/min
6.Pressure setting range1.0–39.2 MPa(10–400kgf/cm2)
7.DetectorUV-VIS Detector
8.Wave length range190–900nm
9.Light SourceDeuterium lamp

Only a 15% of the known compounds lend themselves to analyze by gas chromatography owing to insufficient volatility or thermal instability. Liquid column chromatograph does not have this limitation. The interchange or combination of solvents can provide special selectivity effects that are absent when the mobile phase is a gas. Ionic compounds, liable naturally occurring compounds, polymers and high molecular weight poly functional compounds are conveniently analyzed by liquid column chromatography was achieved by gravity, modern Liquid chromatograph uses high pressure pumps with relatively short, narrow-bore columns containing small particles of packing.